Magnetic Beads:Article Title: Anti-CD3 antibody and molecules comprising the antibody
Article Snippet: 3)-1 cDNA Synthesis Cell lysates (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), and 2.5 mM dithiothreitol (DTT)) of the rat anti-CD3 antibody (C3-147)-producing hybridoma were mixed with oligo dT25-bound magnetic beads of Dynabeads mRNA DIRECT Kit (Thermo Fisher Scientific Inc.) so that the mRNA was bound to the magnetic beads. .. Next, the magnetic beads were washed once each with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, and 0.1% Triton X-100) and a solution for cDNA synthesis (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, and 1.2 units of RNase inhibitor (Thermo Fisher Scientific Inc.). .. Then, a cDNA was synthesized using a solution for cDNA synthesis supplemented with 12 units of SuperScript III Reverse Transcriptase (Thermo Fisher Scientific Inc.).
Article Title: Anti-Orai1 antibody
Article Snippet: 3)-1 cDNA Synthesis A cell lysate (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), 2.5 mM dithiothreitol (DTT)) of the hybridoma producing each antibody R118 or R198 was mixed with magnetic beads (Dynabeads mRNA DIRECT Kit, Invitrogen Corp.) bound with oligo dT25 so that mRNA bound to the magnetic beads. .. Next, the magnetic beads were washed once each with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, 0.1% Triton X-100) and a solution for cDNA synthesis (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by cDNA synthesis using a solution for cDNA synthesis supplemented with 12 units of SuperScript III Reverse Transcriptase (Life Technologies Corp.). .. Subsequently, the magnetic beads were washed with a 3′ tailing reaction solution (50 mM potassium phosphate, 4 mM MgCl2, 0.5 mM dGTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by 3′ tailing reaction using a reaction solution supplemented with 48 units of Terminal Transferase, recombinant (F. Hoffmann-La Roche, Ltd.).
Article Title: Anti-Orai1 antibody
Article Snippet: 3)-1 cDNA Synthesis A cell lysate (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), 2.5 mM dithiothreitol (DTT)) of the hybridoma producing each antibody R118 or R198 was mixed with magnetic beads (Dynabeads mRNA DIRECT Kit, Invitrogen Corp.) bound with oligo dT25 so that mRNA bound to the magnetic beads. .. Next, the magnetic beads were washed once each with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, 0.1% Triton X-100) and a solution for cDNA synthesis (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by cDNA synthesis using a solution for cDNA synthesis supplemented with 12 units of SuperScript III Reverse Transcriptase (Life Technologies Corp.). .. Subsequently, the magnetic beads were washed with a 3′ tailing reaction solution (50 mM potassium phosphate, 4 mM MgCl2, 0.5 mM dGTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by 3′ tailing reaction using a reaction solution supplemented with 48 units of Terminal Transferase, recombinant (F. Hoffmann-La Roche, Ltd.).
Article Title: Anti-GPR20 antibody and anti-GPR20 antibody-drug conjugate
Article Snippet: Rat IgG2a and IgG2b isotype control antibodies (R&D Systems, Inc.) were used as negative control antibodies recognizing an antigen unrelated to GPR20. .. Subsequently, the magnetic beads were washed once each with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, 0.1% Triton X-100) and with a solution for cDNA synthesis (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by cDNA synthesis in a solution for cDNA synthesis to which 12 units of SuperScript III Reverse Transcriptase (Life Technologies Corp.) had been added. .. 3)-1 cDNA Synthesis A cell lysate of each anti-GPR20 antibody-producing hybridoma (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), 2.5 mM dithiothreitol (DTT)) was mixed with oligo dT25-conjugated magnetic beads (Dynabeads mRNA DIRECT Kit, Life Technologies Corp.), so that mRNA was bound to the magnetic beads.
Article Title: Anti-GPR20 antibody and anti-GPR20 antibody-drug conjugate
Article Snippet: Rat IgG2a and IgG2b isotype control antibodies (R&D Systems, Inc.) were used as negative control antibodies recognizing an antigen unrelated to GPR20. .. Subsequently, the magnetic beads were washed once each with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, 0.1% Triton X-100) and with a solution for cDNA synthesis (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by cDNA synthesis in a solution for cDNA synthesis to which 12 units of SuperScript III Reverse Transcriptase (Life Technologies Corp.) had been added. .. 3)-1 cDNA synthesis A cell lysate of each anti-GPR20 antibody-producing hybridoma (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), 2.5 mM dithiothreitol (DTT)) was mixed with oligo dT25-conjugated magnetic beads (Dynabeads mRNA DIRECT Kit, Life Technologies Corp.), so that mRNA was bound to the magnetic beads.
Article Title: Anti-LPS O11 antibody
Article Snippet: 1)-2-2 Synthesis of cDNA The cells individually separated by the cell sorter were dissolved in a cell lysate (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), 2.5 mM dithiothreitol (DTT)) containing magnetic beads (Dynabeads mRNA DIRECT Kit, Invitrogen) bound to oligo dT25, so that mRNA was bound to magnetic beads. .. Then the magnetic beads were washed once with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, 0.1% Triton X-100) and cDNA synthesis solution (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, and 48 units RNase inhibitor (Invitrogen)), respectively, and synthesis of cDNA was performed in a cDNA synthesis solution supplemented with 480 units SuperScript III Reverse Transcriptase (Invitrogen). .. The beads were washed with 3′ tailing reaction solution (50 mM potassium phosphate, 4 mM MgCl2, 0.5 mM dGTP, 0.2% Triton X-100, 48 units RNase inhibitor (Invitrogen)), and the 3′ tailing reaction was carried out in a reaction solution to which 480 units Terminal Transferase, recombinant (Roche) was added.
cDNA Synthesis:Article Title: Anti-CD3 antibody and molecules comprising the antibody
Article Snippet: 3)-1 cDNA Synthesis Cell lysates (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), and 2.5 mM dithiothreitol (DTT)) of the rat anti-CD3 antibody (C3-147)-producing hybridoma were mixed with oligo dT25-bound magnetic beads of Dynabeads mRNA DIRECT Kit (Thermo Fisher Scientific Inc.) so that the mRNA was bound to the magnetic beads. .. Next, the magnetic beads were washed once each with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, and 0.1% Triton X-100) and a solution for cDNA synthesis (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, and 1.2 units of RNase inhibitor (Thermo Fisher Scientific Inc.). .. Then, a cDNA was synthesized using a solution for cDNA synthesis supplemented with 12 units of SuperScript III Reverse Transcriptase (Thermo Fisher Scientific Inc.).
Article Title: Anti-Orai1 antibody
Article Snippet: 3)-1 cDNA Synthesis A cell lysate (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), 2.5 mM dithiothreitol (DTT)) of the hybridoma producing each antibody R118 or R198 was mixed with magnetic beads (Dynabeads mRNA DIRECT Kit, Invitrogen Corp.) bound with oligo dT25 so that mRNA bound to the magnetic beads. .. Next, the magnetic beads were washed once each with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, 0.1% Triton X-100) and a solution for cDNA synthesis (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by cDNA synthesis using a solution for cDNA synthesis supplemented with 12 units of SuperScript III Reverse Transcriptase (Life Technologies Corp.). .. Subsequently, the magnetic beads were washed with a 3′ tailing reaction solution (50 mM potassium phosphate, 4 mM MgCl2, 0.5 mM dGTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by 3′ tailing reaction using a reaction solution supplemented with 48 units of Terminal Transferase, recombinant (F. Hoffmann-La Roche, Ltd.).
Article Title: Anti-Orai1 antibody
Article Snippet: 3)-1 cDNA Synthesis A cell lysate (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), 2.5 mM dithiothreitol (DTT)) of the hybridoma producing each antibody R118 or R198 was mixed with magnetic beads (Dynabeads mRNA DIRECT Kit, Invitrogen Corp.) bound with oligo dT25 so that mRNA bound to the magnetic beads. .. Next, the magnetic beads were washed once each with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, 0.1% Triton X-100) and a solution for cDNA synthesis (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by cDNA synthesis using a solution for cDNA synthesis supplemented with 12 units of SuperScript III Reverse Transcriptase (Life Technologies Corp.). .. Subsequently, the magnetic beads were washed with a 3′ tailing reaction solution (50 mM potassium phosphate, 4 mM MgCl2, 0.5 mM dGTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by 3′ tailing reaction using a reaction solution supplemented with 48 units of Terminal Transferase, recombinant (F. Hoffmann-La Roche, Ltd.).
Article Title: Anti-LPS O11 antibody
Article Snippet: 1)-2-2 Synthesis of cDNA The cells individually separated by the cell sorter were dissolved in a cell lysate (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), 2.5 mM dithiothreitol (DTT)) containing magnetic beads (Dynabeads mRNA DIRECT Kit, Invitrogen) bound to oligo dT25, so that mRNA was bound to magnetic beads. .. Then the magnetic beads were washed once with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, 0.1% Triton X-100) and cDNA synthesis solution (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, and 48 units RNase inhibitor (Invitrogen)), respectively, and synthesis of cDNA was performed in a cDNA synthesis solution supplemented with 480 units SuperScript III Reverse Transcriptase (Invitrogen). .. The beads were washed with 3′ tailing reaction solution (50 mM potassium phosphate, 4 mM MgCl2, 0.5 mM dGTP, 0.2% Triton X-100, 48 units RNase inhibitor (Invitrogen)), and the 3′ tailing reaction was carried out in a reaction solution to which 480 units Terminal Transferase, recombinant (Roche) was added.
Reverse Transcription:Article Title: Anti-Orai1 antibody
Article Snippet: 3)-1 cDNA Synthesis A cell lysate (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), 2.5 mM dithiothreitol (DTT)) of the hybridoma producing each antibody R118 or R198 was mixed with magnetic beads (Dynabeads mRNA DIRECT Kit, Invitrogen Corp.) bound with oligo dT25 so that mRNA bound to the magnetic beads. .. Next, the magnetic beads were washed once each with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, 0.1% Triton X-100) and a solution for cDNA synthesis (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by cDNA synthesis using a solution for cDNA synthesis supplemented with 12 units of SuperScript III Reverse Transcriptase (Life Technologies Corp.). .. Subsequently, the magnetic beads were washed with a 3′ tailing reaction solution (50 mM potassium phosphate, 4 mM MgCl2, 0.5 mM dGTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by 3′ tailing reaction using a reaction solution supplemented with 48 units of Terminal Transferase, recombinant (F. Hoffmann-La Roche, Ltd.).
Article Title: Anti-Orai1 antibody
Article Snippet: 3)-1 cDNA Synthesis A cell lysate (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), 2.5 mM dithiothreitol (DTT)) of the hybridoma producing each antibody R118 or R198 was mixed with magnetic beads (Dynabeads mRNA DIRECT Kit, Invitrogen Corp.) bound with oligo dT25 so that mRNA bound to the magnetic beads. .. Next, the magnetic beads were washed once each with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, 0.1% Triton X-100) and a solution for cDNA synthesis (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by cDNA synthesis using a solution for cDNA synthesis supplemented with 12 units of SuperScript III Reverse Transcriptase (Life Technologies Corp.). .. Subsequently, the magnetic beads were washed with a 3′ tailing reaction solution (50 mM potassium phosphate, 4 mM MgCl2, 0.5 mM dGTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by 3′ tailing reaction using a reaction solution supplemented with 48 units of Terminal Transferase, recombinant (F. Hoffmann-La Roche, Ltd.).
Article Title: Anti-GPR20 antibody and anti-GPR20 antibody-drug conjugate
Article Snippet: Rat IgG2a and IgG2b isotype control antibodies (R&D Systems, Inc.) were used as negative control antibodies recognizing an antigen unrelated to GPR20. .. Subsequently, the magnetic beads were washed once each with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, 0.1% Triton X-100) and with a solution for cDNA synthesis (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by cDNA synthesis in a solution for cDNA synthesis to which 12 units of SuperScript III Reverse Transcriptase (Life Technologies Corp.) had been added. .. 3)-1 cDNA Synthesis A cell lysate of each anti-GPR20 antibody-producing hybridoma (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), 2.5 mM dithiothreitol (DTT)) was mixed with oligo dT25-conjugated magnetic beads (Dynabeads mRNA DIRECT Kit, Life Technologies Corp.), so that mRNA was bound to the magnetic beads.
Article Title: Anti-GPR20 antibody and anti-GPR20 antibody-drug conjugate
Article Snippet: Rat IgG2a and IgG2b isotype control antibodies (R&D Systems, Inc.) were used as negative control antibodies recognizing an antigen unrelated to GPR20. .. Subsequently, the magnetic beads were washed once each with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, 0.1% Triton X-100) and with a solution for cDNA synthesis (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, 1.2 units of RNase inhibitor (Life Technologies Corp.)), followed by cDNA synthesis in a solution for cDNA synthesis to which 12 units of SuperScript III Reverse Transcriptase (Life Technologies Corp.) had been added. .. 3)-1 cDNA synthesis A cell lysate of each anti-GPR20 antibody-producing hybridoma (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), 2.5 mM dithiothreitol (DTT)) was mixed with oligo dT25-conjugated magnetic beads (Dynabeads mRNA DIRECT Kit, Life Technologies Corp.), so that mRNA was bound to the magnetic beads.
Article Title: Anti-LPS O11 antibody
Article Snippet: 1)-2-2 Synthesis of cDNA The cells individually separated by the cell sorter were dissolved in a cell lysate (50 mM Tris-HCl (pH 7.5), 250 mM LiCl, 5 mM EDTA (pH 8), 0.5% lithium dodecyl sulfate (LiDS), 2.5 mM dithiothreitol (DTT)) containing magnetic beads (Dynabeads mRNA DIRECT Kit, Invitrogen) bound to oligo dT25, so that mRNA was bound to magnetic beads. .. Then the magnetic beads were washed once with mRNA washing solution A (10 mM Tris-HCl (pH 7.5), 0.15 M LiCl, 1 mM EDTA, 0.1% LiDS, 0.1% Triton X-100) and cDNA synthesis solution (50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 5 mM DTT, 0.5 mM dNTP, 0.2% Triton X-100, and 48 units RNase inhibitor (Invitrogen)), respectively, and synthesis of cDNA was performed in a cDNA synthesis solution supplemented with 480 units SuperScript III Reverse Transcriptase (Invitrogen). .. The beads were washed with 3′ tailing reaction solution (50 mM potassium phosphate, 4 mM MgCl2, 0.5 mM dGTP, 0.2% Triton X-100, 48 units RNase inhibitor (Invitrogen)), and the 3′ tailing reaction was carried out in a reaction solution to which 480 units Terminal Transferase, recombinant (Roche) was added.
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